Making mice from induced pluripotent stem cells and reducing functional diversity among stem cell lines

In 2009, our lab produced the first iPSCs that were able to generate entire mice using tetraploid complementation. Later experiments showed that these lines harbored relatively few structural mutations. Together these experiments supported the notion that these cells would be safe and useful. Continuing studies aim to improve the reliability of the lines and assess the contribution of somatic mutation and epigenetic abnormalities to functional diversity and unwanted potential tumorigenicity of the lines.